Protein_Domain

Part:BBa_K4190004

Designed by: Zoe L. Petroff   Group: iGEM22_UCSC   (2022-09-19)


10aa GS Linker (E. coli)

Links His-tag to Exendin-4 protein. Codon optimized for E. coli.

Usage and Biology

We used a ten amino acid linker of alternating glycine and serines to distance our His-tag from our polypeptide, and enhance His-tag exposure for protein purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//proteindomain/linker
Parameters
None